ScreeningacDNALibrary
Screening a cDNA Libraryfor use with HybriZAP zebrafish cDNA librariesObjectivecDNA library screening allows detection of expressed genes for subsequent cloning and sequencing.Selecting a LibraryCurrently, our lab has access to cDNA libraries from three developmental time points.Maternal: messages from embryos before midblastula-transistion, when zygotic transcription begins (~0-3 hours of development).Shield: messag......閱讀全文
Screening－a－cDNA－Library
Screening a cDNA Libraryfor use with HybriZAP zebrafish cDNA librariesObjectivecDNA library screening allows detection of expressed genes for subseque
cDNA－LIBRARY－SCREENING
PREPARE SOLUTIONS1. 10mM MgSO4, 0.2% Maltose LB (100 mL):Mix?1.0?g of Bacto-Tryptone,?1.0?g of NaCl,?0.5?g of Yeast Extract, and?1.0?mL of 1M MgSO4. A
Library－cDNA－Synthesis
Library cDNA Synthesis1° cDNA SynthesisN.B:?During 1° cDNA synthesis, all steps should be carried while wearing gloves and all solutions should either
Differential－cDNA－Screening－Procedures
Differential cDNA Screening ProceduresThe protocols listed refer to cDNA library construction and preliminary differential screening procedures. They
Experimental－Protocol－for－cDNA－Library－Construction
Experimental Protocol for cDNA Library ConstructionIdentify appropriate celltype over-expressing corresponding gene.Find out if transcription can be s
Expression－Library－Screening－(Procaryotic)－Using－APFusion－Proteins
Outline:Bacteriophage lambda is a linear double stranded DNA, approximately 50 kB in size. The two sticky ends help the phage to recircularize after e
cDNA－AMPLIFICATION－FROM－LAMBDAPHAGE－LIBRARY
PREPARE SOLUTIONS1. SM buffer (1 L):Mix?5.8?g of NaCl,?2?g of MgSO4-7H2O,?50?mL of 1M Tris-HCl, pH 7.5,?0.5?mL of 2% gelatin, and dH2O to 1 L (Autocla
Microaspiration－of－Esophageal－Gland－Cells－and－cDNA－Library－Construction－for
Microaspiration of Esophageal Gland Cells and cDNA Library Construction for Identifying Parasitism Genes of Plant-Parasitic NematodesIdentifying p
CDNA文庫
?CDNA文庫（主要內容如下）·?????????Construction of cDNA Library·?????????Construction of Genome DNA Library·?????????Library Screening??OthersConstruction of cD
cDNA
·?????????cDNA Synthesis?(Crawford Lab)mRNA can be converted into DNA (copy DNA, cDNA) by annealing oligo-dT to the 3' poly-A tail that occurs on
細菌人工染色體
The Construction of Bacterial Artificial Chromosome (BAC) Libraries (complete manuscript)?(Clemson University Genomics Institute)??Construction of BAC
How－to－build－a－BAC－library
Introduction???The?most?important?aspect??of?our?cloning??vectors?is?that?they?are based?on???the?E.?coli?F-factor???replicon.?It?allows?for??strict?
Organelle－DNA－Library－Construction
Organelle DNA Library Construction(version MAY-1998)I.?NEBULIZATION?of?DNA ?????1.?0.5?-?5?ug?DNA?in?TE?(10mM/1mM),?25%?glycerol,?final?volume?500?ul
高通量篩選：化合物庫在藥物研發過程中的應用MedChe...2
二、化合物庫使用方法及使用注意事項? 化合物庫篩選過程主要包括化合物庫選擇、實驗方案設計、實驗進行、實驗結果檢測、實驗方案優化等步驟。進行化合物庫篩選，要根據自己的實驗目的選擇合適的化合物庫，設計適合的實驗方案，并設計好化合物作用結果的檢測指標及檢測方法。化合物庫篩選的實驗方案設計至少需要包括以下
蛋白質相互作用
Interaction Trap/Trap Two-Hybrid System·?????????Yeast Two-Hybrid System?(Finley Lab)This is one of the most comprehensive and detailed guide to yeast
Chloroplast－Phenomics:－Systematic－Phenotypic－Screening－of－Chloroplast－...
Chloroplast Phenomics: Systematic Phenotypic Screening of Chloroplast Protein Mutants in ArabidopsisAs part of a project to analyze chloroplast functi
Construction－of－BAC－Libraries：Construction－of－a－BAC－library
Once high molecular weight (HMW) DNA has been prepared it must somehow be fragmented and DNA in the desired size range isolated. In general, as the de
Screening－BAC－filters－with－nonradioactive－probes
(Protocol for a single high-density BAC colony filter (HDR filter) of 22 cmx22 cm) to be screened using Amersham''s ECL hybridization kit; thi
酵母人工染色體
·?????????Easy YAC Preparation Method?(Andrew Davies,Shaw lab)·?????????Screening YAC libraries?(Donis Keller Lab)This is a method for screening YAC l
Construction－of－BAC－Libraries：SOLUTIONS－FOR－BAC－LIBRARY－CONSTRUCTION
SOLUTIONS FOR BAC LIBRARY CONSTRUCTION10X Homogenization Buffer (HB) stock: (1 liter)IngredientAmountFinal ConcentrationTrisma base12.1 g0.1 MKCl59.7
Easy－Way－to－Clone－Genes－From－a－Phage－Library
Easy Way to Clone?The protocol is oriented towards a C. albicans genomic library I made in Lambda Zap II on 7/97.The overall sequence of events is:??
植物表型成像系統WIWAM－Screening功能簡介
WIWAM Screening植物表型野外樣帶成像分析系統由野外移動式植物表型組學成像分析平臺、RGB成像、葉綠素熒光成像、高光譜成像、植物紅外熱成像、植物近紅外成像等組成，移動式成像分析平臺具輪子，可以沿軌道滑行并對植物進行表型組成像分析；各成像分析單元為模塊式結構，可靈活安裝配置到移動式成像
cDNA－Libraries
cDNA LibrariesIsolation of corresponding genetic informationInstead of synthesizing a desired gene, can we used the amino acid information to directly
Screening－Bacterial－Colonies－Using－Xgal－and－IPTG:－αComplementation
實驗概要? ? ? ? α-complementation occurs when two inactive fragments of E. coli β-galactosidase associate to form a functional?enzyme. Many plasmid
植物表型成像系統WIWAM－Screening功能分析
紅外熱成像分析（選配）：用于成像分析植物在光輻射情況下的二維發熱分布，良好的散熱可以使植物耐受較長時間的高光輻射或低水條件（干旱） 近紅外成像分析（選配）：用于觀測分析植物的水分狀態及其在不同組織間的分布變異，處于良好澆灌狀態的植物表現出對近紅外光譜的高吸收性，而處于干旱狀態的植物則表現出對近
人VL基因文庫（genomic－library）的構建
[器材和試劑]?● PCR試劑和設備?● cFv基因文庫單鏈模板DNA,制備自pHENl中的天然scFv文庫（10ng/u1）?● Gelleclean試劑盒（Qbiogene）?● Wtzard PCR純化試劑盒（ProlneSa）?● RJHl/2Xho引物： 5'-GGC ACC CT
cDNA文庫構建技術cDNA鏈的反轉合成
構建cDNA文庫是一種獲得目標基因并進一步進行基因重組,基因克隆的重要方法,而在該文庫構建中有mRNA 逆轉錄出cDNA是一步極為關鍵的操作。mRNA的逆轉錄主要由MMLV、AMV兩種逆轉錄酶催化合成cDNA，在這個逆轉錄過程中，模板mRAN及逆轉錄酶是兩個最重要的影響因素。對于mRNA來講，一
構建cDNA文庫的層析柱cDNA分級
這一步稍不注意會影響成功性或影響獲得的cDNA的片段分布特點。這一步的操作要小心，尤其要在加入cDNA之前通過反復懸浮和試滴保證柱子能正常工作，cDNA的加入和收集要精力集中。獲得的每一級的cDNA量很少，檢測時帶型很暗，所以要用新鮮做的透明薄膠檢測，根據檢測結果一定要舍棄太短的cDNA（一般4
cDNA文庫構建技術cDNA鏈的反轉合成
實驗概要構建cDNA文庫是一種獲得目標基因并進一步進行基因重組，基因克隆的重要方法，而在該文庫構建中有mRNA 逆轉錄出cDNA是一步極為關鍵的操作。mRNA的逆轉錄主要由MMLV、AMV兩種逆轉錄酶催化合成cDNA，在這個逆轉錄過程中，模板mRAN及逆轉錄酶是兩個最重要的影響因素。對于mR
運用肽庫篩選磷酸化激酶motif方法
Screening Kinase Phosphorylation Motifs Using Peptide LibrariesIsaac A. Manke and Michael B. YaffeCenter for Cancer Research, Massachusetts Institute