Development(Immuno...
實驗概要Peprotech provides a development (immunostaining) protocol.實驗步驟1. After the transfer is complete, incubate the membrane in blocking solution (3% Nonfat Dry Milk in diH2O) for 30 minutes with gentle agitation on an orbital shaker.2. Wash the membrane three times with TBST (TBS, pH 7.2 with 0.1% TWEEN-20) in a clean tray on an orbital shaker; each wash lasting 5-10 minutes.3. Dilute the probing (......閱讀全文
Development－(Immuno...
實驗概要Peprotech provides a development (immunostaining) protocol.實驗步驟1.?After the transfer is complete, incubate the membrane in blocking solution (3% N
Hop－Pathway－in－Cardiac－Development
Homeodomain transcription factors comprise a large family of DNA binding factors that regulate transcription and development. Many homeodomain genes a
Melanocyte－Development－and－Pigmentation－Pathway
Studies with homozygous knockout mice have suggested that stem cell factor (SCF or KITLG), c-Kit, microphthalmia (Mitf), and B-cell CLL/lymphoma 2 (Bc
Solutions－for－Sea－Urchin－Development
Solutions for Sea UrchinASW: Artificial sea water (MBL)NaCl24.72 gKCl0.67 gCaCl2.2H2O1.36 gMgCl2.6H2O4.66 gMgSO4.7H2O6.29 gNaHCO30.18 gdH2Oto 1 literS
The－Effects－of－Ethanol－on－Zebrafish－Development
Introduction:The early stages of zebrafish,?Danio rerio?development are characterized by meroblastic dicoidal cleavage. Initially cell division only o
Effect－of－ethanol－on－development－of－Danio－reriro
ObjectiveThe objective of the experiment is to determine the effects of ethanol exposure on the embryonic development of zebrafish through observation
Development－of－the－Heart－Morphogenetic－Field－in－the－Axolotl－Embryo
IntroductionThere are two modes of development common to most species in the animal kingdom.Virtually all embryos undergo both mosaic and regulative d
The－effect－of－temperature－on－the－embryonic－development－of－sea－urchins
Objective:?This experiment aims to discover if temperature has an effect on the rates of sea urchin embryonic development. By altering the external en
Regulative－Development－in－Axolotl－Embryos;－Splitting－the－Heart－Field
ObjectiveThis experiment will seek to demonstrate regulative development in?Ambystoma mexicanum?embryos. Specifically, we will split the morphogenetic
Lissencephaly－gene－(LIS1)－in－neuronal－migration－and－development
Integration of pathways that regulate nucleokinesis during neuronal migration and a model of LIS1 mediating CLIP-170 interactions with the dynein/dyna
Early－development－of－primary－motor－neurons－and－somites－in－Zebrafish－Embryos
Background:Zebrafish,or the teleost fish Danio rerio,is a rapidly developing organism that is apopular species for studying vertebrate development. Cl
BCEIA2019－International－Summit－on－Scientific－Instruments－Development
Contact PersonMs. Liying WangPhone:+86-10-6851 2289E-mail: liying.wang@caia.org.cnAbout BCEIA:Sponsored by CAIA (China Association for Instrumental
Effect－of－UV－Treatment－on－Early－Development－of－Sea－Urchin－Embryos
Objective:To test the effects of UV radiation at 254nm on Sea Urchin embryo development after radiation at the 2-cell stage.Background:Sea Urchins exh
FGFR－INHIBITOR,－SU5402,－ON－CRANIOFACIAL－DEVELOPMENT－IN－CHICKEN－EMBRYOS
ABSTRACTFibroblast growth factor (FGF) signaling plays a signifigant role in embryonic development. Particularyl, FGFs are implicated in mediating epi
Development：懷孕期間飲酒的危害研究
孕期飲酒會對正在發育的嬰兒產生毀滅性的影響，導致認知和行為不良。酒精還會增加流產、死產和其他胎盤并發癥的風險。但是如果一個母親在她知道自己懷孕之前就喝酒了呢？英國、美國和澳大利亞的健康指南稱，懷孕期間最好避免飲酒。但由于缺乏科學證據，科學家提出這一建議的時候還是很謹慎，所以女性對此不屑一顧是很常
記憶力下降？或與它有關－|－Genes－－Development
1月31日，發表在《Genes and Development》期刊上的這篇最新論文揭示了這一衰退的關鍵原因：無論是線蟲還是人類，大腦一關鍵物質——犬尿喹啉酸（kynurenic acid，KYNA）會隨著年齡的增長而累積。而且，犬尿喹啉酸會進一步干擾谷氨酸（對于記憶、學習等必需的一種大腦化合物
Look－Ma,－No－Archenteron!－Sulfates－role－in－sea－urchin－early－development
ObjectiveTo observe the role sulfate plays in sea urchin gastrulation, and to replicate the findings of Karp and Solursh, that sea urchin embryos fail
Dendritic－cells－in－regulating－TH1－and－TH2－Development
While T cells and B cells carry out the actions of antigen-specific immune responses, antigen-presenting cells called dendritic cells are required for
Stem－Cells－and－Development－：大腦類器官的新型培育方法
加州大學圣地亞哥分校醫學院的研究人員在當前在線期刊《Stem Cells and Development》雜志上撰文，描述了一種快速，經濟有效的從原代細胞中制造人皮層類器官的方法。 發展人腦功能的實驗研究是有限的，這是由于活胚胎受試者的研究受到倫理問題和大腦本身脆弱性的限制，動物模型僅部分模仿
BioSystem－Development與安捷倫聯手推出微量色譜系統
BioSystem Development 與安捷倫科技聯手推出高精度、高通量的AssayMAP? 微量色譜系統 2010年12 月8 日，北京——BioSystem Development 和安捷倫科技公司（紐約證交所：A）共同發表了一份合作協議，在成熟的安捷倫 Bravo 自動液體
The－Effects－of－CalciumFree－Seawater－onthe－Development－ofSea－Urchin－Embryos
ObjectiveThe purpose of this experiment is to determine the effects of calcium-free seawater on the development of sea urchin embryos. Embryos were pl
Development：揭示心臟發育過程中的細胞信號
在心臟發育的后期，心內膜(細胞的內層)和心肌(心肌)之間的相互作用是至關重要的。而在心臟發育的最初階段，這兩個細胞層之間的信號傳遞一直比較難研究。圖片來源：American Heart Association 現在，醫學博士H. Scott Baldwin和他的同事建立了一個模型，在體外探索心
Development：調控干細胞分化生成β細胞的分子機制
Wnt/β-catenin信號通路和microRNA 335幫助干細胞分化形成祖細胞。這些細胞定位于中胚層，是不同組織類型包括胰腺和β細胞的來源。Helmholtz Zentrum München科學家們發現干細胞分化的關鍵分子功能，可用于β細胞替代治療糖尿病。這兩項研究的結果發表在De
《Development》：英科學家首次成功再生活體器官
最近，愛丁堡大學的一個科學家小組第一次成功地再生了一個活體器官。該研究小組重建了胸腺——人體中產生重要免疫細胞的器官，位于心臟旁邊。 免疫修復 這一進展，為免疫系統受損和影響胸腺發育的遺傳病患者的新療法，奠定了基礎。 研究小組重新激活了一種隨年齡增長而關閉的自然機制，使很老的小
Development：科學家揭示干細胞生境的特殊結構
近日，一項刊登在國際雜志Development上題為“Wnt6 maintains anterior escort cells as an integral component of the germline stem cell niche”的研究報告中，來自范德堡大學的研究人員通過研究闡明了干
Development：利用改進的CRISPR基因編輯平臺研究人類發育
在一項新的研究中，來自英國韋爾科姆基金會桑格研究所和劍橋大學的研究人員構建出一種更加高效的和可控的CRISPR基因組編輯平臺：sOPTiKO。他們描述了這種免費獲得的單步驟系統如何在體內每個細胞和每個發育步驟中發揮作用。這種新方法將有助科學家們開展發育生物學、組織再生和癌癥研究。相關研究結果發表
Development：一種能阻止性別反轉的關鍵基因
生物通報道：來自中科院遺傳與發育生物學的研究人員發表了題為“Translation repression by maternal RNA binding protein zar1 is essential for early oogenesis in zebrafish”的文章，發現斑馬魚zar
Genes－－Development：USTC復合物在piRNA轉錄中的功能
這一研究在線蟲中發現了一個參與piRNA轉錄過程的上游序列轉錄復合物（USTC復合物）。 piRNA是一類在動物中保守的非編碼小RNA，在轉座子沉默、生殖發育、基因表達調控和性別決定等過程中發揮重要作用。 來自中國科學技術大學生命科學學院光壽紅課題組與劍橋大學的Eric Miska課題組合作
Development發表上海生科院關于精原細胞分化新發現
3月10日，國際學術期刊Development 在線發表了中國科學院上海生命科學研究院生物化學與細胞生物學研究所童明漢研究組的最新研究成果：Retinoid signaling controls spermatogonial differentiation by regulating expre
IL12－and－Stat4－Dependent－Signaling－Pathway－in－Th1－Development
Interleukin-12 (IL-12) promotes cell-mediated immunity by inducing Th1 cell differentiation and activation of both T cells and NK cells. Dendritic cel