Multiplestudieswithasingleexperiment:ThePowerof...（一）
Multiple studies with a single experiment: The Power of Quantitative MultiplexingMultiple studies with a single experiment: The Power of Quantitative Multiplexing Overview Part1：IntroductionAn overview of multiplexed isobaric labeling, the basics of Tandem Mass Tags and the SPS MS3 technique Part2：Sample Prep A summary of the steps involved for a complete TMT workflow including labeling and ......閱讀全文
Multiple－studies－with－a－single－experiment:－The－Power－of－－...（一）
Multiple studies with a single experiment: The Power of Quantitative MultiplexingMultiple studies with a single experiment: The Power of Quantitative
Multiple－studies－with－a－single－experiment:－The－Power－of－...（四）
Better Ion Transmission With Segmented Quadrupole?Segmented Quadrupole ?Improved transmission across m/z range and for narrow windows?Brighter Source
Multiple－studies－with－a－single－experiment:－The－Power－of－...（六）
??SCX and high pH reversed phase fractionation are both orthogonal to low pH C18 LC separation?Strong cation exchange (SCX) requires sample desalting
Multiple－studies－with－a－single－experiment:－The－Power－of－...（七）
SPS TMT Method Development- What is New on Lumos?Multiplexing On the Benchtop Orbitrap System (Q Exactive Series)??Take advantage of segmented quadrup
Multiple－studies－with－a－single－experiment:－The－Power－of－...（三）
Ratio Distortion with Isobaric MultiplexingProblem: Quantitation of low-abundance proteins in a complex background is distorted by co-isolated interfe
Multiple－studies－with－a－single－experiment:－The－Power－of－...（二）
A Better Multiplexing Method– Isobaric Mass Tagging??Less MS1 Complexity?????Increased Throughput Concurrent MS analysis of multiple samples?????Less
Multiple－studies－with－a－single－experiment:－The－Power－of－...（五）
Competitive Advantages?Trust your quantitation!?Multinotch MS3 quantitation is more accurate than other MS2 Methods?The accuracy of Multinotch MS3 qua
Dual－and－TripleCo...－（一）
實驗概要Cytokine ELISPOT ?has become a powerful routine tool for the analysis of disease- as well ?as vaccine-induced T-cell responses. The method is limi
Fluidigm公司微液流芯片在單細胞研究中的應用（二）
It was also worth it for Tannishtha Reya, who studies stem cell fate and cancer at Duke University Medical Center in Durham, North Carolina. "Im
全基因組的比較基因組雜交技術介紹
Whole-Genome and Custom Fine-Tiling Array CGHComparative Genomic Hybridization (CGH) measures DNA copy number differences between a reference genome a
WBand－FrequencySwept－EPR（一）
W-Band Frequency-Swept EPR毫米波（W頻段 75-110GHz）頻率掃描EPR系統實驗James S. Hyde,a,*?Robert A. Strangeway,a,b?Theodore G. Camenisch,a?Joseph J. Ratke,a?and?Wojcie
Low－Loss－Sapphire－Windows－for－High－Power－Microwave－Transmission（一）
Dr. Stephen C. Bates，Thoughtventions Unlimited LLC40 Nutmeg Lane??Glastonbury, CT 06033EXECUTIVE SUMMARYThe Problems.Windows that transmit high powe
Heterogeneity－of－SingleCell－Gene－Expression－Across－Phenotypically（一）
Introduction? Multi-cellular ?populations are fundamentally driven by the collective properties of ?individual cells. However, our understanding of ge
單細胞測序技術(single－cell－sequencing)－綜述（一）
單細胞生物學最近幾年是非常熱門的研究方向。在這一領域中，最前沿的則是單細胞測序技術。傳統測序方法一次處理成千上萬個細胞，得到的變異水平也是成千上萬個細胞的平均后水平。但是，就如同世界上沒有完全相同的兩片樹葉一樣，沒有兩個細胞是完全相同的。所以，單細胞測序對于研究單個細胞就顯得至關重要。單細胞測序可以
Streaking－Bacteria－for－Single－Colonies
1. Initial inoculum:- From solid media, touch an isolated colonies with a sterile applicator or toothpick.- From liquid media, touch the culture with
Multiple－Tissue－Northern－(MTN#8482;)Blots
人、小鼠和大鼠的多種組織來源的高質量mRNA,經電泳分離后預轉于尼龍膜上預制的即用型Northern雜交膜，免去RNA電泳操作過程可檢測范圍：0.5-10kb可重復使用提供ExpressHybTM快速雜交液樣品可靠的優良品質從1991年始，公司就向廣大的生命科學研究者提供高質量的預制雜交膜。而MTN
Molecular－Analysis－and－Results－－DNA
Theory of CGHComparative genomic hybridization (CGH) is a fairly new molecular cytogenetic technique that allows detection of DNA sequence copy number
T－cell－Activation－Protocol
IntroductionMature T cells recognize and respond to the antigen/MHC complex through their antigen-specific receptors (TCR). The most immediate consequ
SingleCell－Electroporation－in－Xenopus
Single-Cell Electroporation in XenopusXue Feng Liu and Kurt HaasINTRODUCTIONSingle-cell electroporation (SCE) is a versatile technique for delivering
single－atom－tips－(SATs)制作
目前普遍制作的SATs的方法主要有兩種：（1）構建法（2）選擇性氣蝕法。
Single－tube－confirmation－PCR－protocol
The following colony PCR protocol has been designed to be performed in individual reaction tubes. We usually test three colonies from each transformat
Agarose－Gels－for－Single－Stranded－DNA
1. Prepare 50X TAE as:242 g Tris Base57.1 mL Glacial Acetic Acid100 mL 500 mM EDTA, pH 8.0600 mL ddH2OMix. Bring volume to 1 L. Autoclave.2. Mix the f
Laser－Capture－Microdissection－(LCM)
This method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor
Recommended－Experiments－with－Isolated－Mitochondria
In our teaching lab we encourage students to work with each other and to share insight, experience, and even experimental results. To facilitate such
SingleVirus－Tracking－in－Live－Cells
Single-Virus Tracking in Live CellsMichael J. Rust, Melike Lakadamyali, Boerries Brandenburg and Xiaowei Zhuang?INTRODUCTIONReal-time, live-cell imagi
SNPs(single－nucleotide－polymorphism)的概念
SNPs(single nucleotide polymorphism ， SNP ，發音為 “snips”), 主要是指在基因組水平上由單個核苷酸的變異所引起的 DNA 序列多態性。它是人類可遺傳的變異中最常見的一種。占所有已知多態性的 90% 以上。 SNP 在人類基因組中廣泛存在，平均
Single－Primer－(SemiRandom)－PCR
DescriptionSingle primer PCR allows amplification from known to unknown regions in chromosomes, phage, plasmids, large PCR products and other sources
Size－and－Shape－of－Protein－Molecules4
Determining the Molecular Weight of a Protein Molecule—Combining?S?and?R?s?à la Siegel and MonteWith the completion of multiple genomes and increasing
QRTPCR
Comparison of normalisation methodsThere is an ongoing debate what is the best way to normalise qPCR?data. Reference genes are the most common method,
QRTPCR
Comparison of normalisation methodsThere is an ongoing debate what is the best way to normalise qPCR data. Reference genes are the most common method,