SilverStainingProtocol
1x 40min - overnight 50% MeOH, 12% Acetic Acid1x 30min 50% MeOH, 12% Acetic Acid, 0.05% 37% Formaldehyde3x 20min &nbs......閱讀全文
Silver－Staining－Protocol
1x 40min - overnight?????50% MeOH, 12% Acetic Acid1x 30min??????????????????????????????????50% MeOH, 12% Acetic Acid, 0.05% 37% Formaldehyde3x 20min?
SSR－GEL－and－Silver－Staining－Protocol
I.?EQUIPMENT:DNA sequencing unit (35 x 45 cm) & 2000V power supplyClampsLg. plastic trays (4), about 43 x 50 x 8 cm, and one lidTwo rocking platformsH
Intracellular－Staining－Protocol
1.?Fix cells- Add16% formaldehyde directly into culture medium to obtain a final concentration of 1.5% formaldehyde.2. Incubate in fixative for 10 min
銀染(silver－staining)操作規程
實驗原理：在堿性條件下，用甲醛將蛋白帶上的硝酸銀（銀離子）還原成金屬銀，以使銀顆粒沉積在蛋白帶上。染色的程度與蛋白中的一些特殊的基團有關，不含或者很少含半胱氨酸殘基的蛋白質有時候呈負染。銀染的詳細機制還不是非常清楚。 試劑：乙醇、冰醋酸、乙酸鈉、硫代硫酸鈉、硝酸銀、碳酸鈉、甘氨酸或EDTA.Na
Direct/Indirect－Staining－Protocol
Use this Protocol for Directly or Indirectly staining cells for Flow Cytometric Analysis1) Dilute cells to 5x10^6 cells/mL2) Aliquot 100uL of cells pe
Intracellular－Cytokine－Staining－Protocol
實驗概要A ?modification of the basic immunofluorescence staining and flow ?cytometric analysis protocol can be used for the simultaneous analysis ?of surf
Actin－StainingActin－Staining－Protocol
實驗概要Invitrogen ?offers several fluorescent and biotinylated phalloidin and phallacidin ?derivatives for labeling F-actin. These phallotoxins, isolated
Cell－Surface－Immunofluorescence－Staining－Protocol
實驗概要A method of identifying ?and enumerating specific cell types in a heterogeneous population of ?cells by enhancing the specific staining of desired
Methylene－Blue－DNA－staining－protocol
Methylene Blue DNA staining protocolProtocol:Load 2-5X the amount of DNA that would give bands of moderate intensity on an ethidium bromide stained ge
Cell－Cycle－Staining－ProtocolDAPI
1. Harvest cells- wash 2X in PBS to get rid of serum proteins. 1200rpm, 5 min2. Resuspend pellet (up to 3x106 cells) in 1.2 ml PBS (Ca and Mg free).3.
Protocol－for－intracytoplasmic－staining－of－cytokines－for－FACS－analysis
DescriptionProtocol for intracytoplasmic staining of cytokines for FACS analysis?Procedure1) Prepare spleen, lymph node or T cell clone cells as singl
蛋白質電泳
蛋白質電泳（主要內容如下）One-Dimensional SDS-PAGETwo-Demensional SDS-PAGEProtein Electrophoresis in Agarose Gel?Gel StainingRecipesOne-Dimensional SDS-PAGE·??????
低背景的蛋白質銀染(silver－staining)方法
我們做蛋白質電泳的人都知道，銀染色很靈敏，有很強的說服力，但通常膠背景較深，不易掃描。在這里介紹一下低背景的蛋白質銀染方法。Step Reagent Time/minFix 50%EtOH, 12%HAC, 0.1%HCHO, 38%H2O 60Rinse 50% EtOH 5 min, 3 tim
蛋白質檢測
·?????????Protein detection?(Aberdeen's Lab)The method used to locate the proteins following 2D-PAGE depends on the nature of the original sample.
基因型分析
Randomly Amplified Polymorphic DNA (RAPD)Randomly Amplified Polymorphic DNA (RAPD)?by??(DNA KAFFE)RAPD analysis has been successfully used in mapping
Silver－Acetate－Autometallography－(AMG)
In the early eighties, a series of papers were published by Gorm DANSCHER, Aarhus, Denmark, to introduce a reliable and easy-to-handle technique for l
Silver－Enhancement－...
實驗概要The method provides a silver enhancement protocol for immunoassay.主要試劑Prepare the following reagents fresh daily except for the citrate buffer.1.
免疫細胞化學
Introduction to Immunocytochemistry?(House Ear Institute)A brief overview of common available methods.??BrDU Immunocytochemistry using peroxidase and
Protein－Staining－Procedures
This method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor
PVDF膜上蛋白的可逆染色
PVDF膜上蛋白的可逆染色???Western雜交時，為確認蛋白是否轉至PVDF膜上，可用下列方法對膜上蛋白進行可逆性染色。1.??????氨基黑染色染液：0.5% Amido Black (w/v), 25% isopropanol (v/v) and 10% acetic acid.染色：將PV
蛋白質電泳技術2
Decrease toxicity of destain even more!The destain procedure can be made even less toxic by replacing the destaining solution completely with MilliQ w
丙烯酰胺膠中肽提取和蛋白消化技術
Digestion of Proteins and Extraction of Peptides from an Acrylamide GelSherry Niessen, Ian McLeod and John R. Yates IIIDepartment of Cell Biology, The
組織學——包埋技術
Tissue Preparation for Methy Methacrylate EmbeddingMethyl Methacrylate Embedding Protocols?(Energy Beam Sciences, Inc.)General protocol for plastic em
Silver:－Lysate－for－Western
Grow cellsHarvestSpin downWash with PBSSpin down enough cells to collect a 50-100 μL cell pellet in a FastPrep tubePrepare lysis buffer (beforehand) a
Silver:－Colony－PCR
Zymolyase Solution:Regular stock of zymolyase is 10 mg/ml diluted in water, mix by inverting, not all will go into solution (filter it) store aliquots
組織學——染色
Hematoxylin and Eosin Staining of Tissue for LCM?(Arcturus)???Immunohistochemical Staining (IHC)?(Arcturus)For optimal LCM from IHC samples, it is nec
Silver:－TimeLapse－Microscopy
Pad Preparation1. Microwave 2% agarose (mix of low-melt and normal, to taste) in Thorn media (see below). (If you have used different percentages of a
細胞遺傳學——原位雜交(ISH)
In Situ Hybridization· ????????In Situ Hybridization?(jsmith1@po-box.mcgill.ca)In situ?hybridization, as the name suggests, is a method of localizing,
免疫組織化學
· ????????Double Peroxidase (HRP) Immunohistochemical Labeling of Trypsin-Sensitive Antigens?(KPL)·?????????Immunohistochemistry?(Tyner lab)This is a
peptide－fingerprint－mapping
Polyacrylamide gel electrophoresis is a widely used technique to separate proteins from biological samples. Moreover, the development of two-dimension