EthanolprecipitationmethodforpurifyingPCRproducts
1. For each PCR reaction (50 μL) prepare a 1.5mL microcentrifuge tube containing the following: - 5 μL of 3M sodium acetate (NaOAc), pH 4.6 - 100 μL of 95% ethanol (EtOH) 2. Pipet the entire contents of each PCR reaction into a tube of sodium acetate/ethanol mixture. Mix throughly. 3. Vortex the tubes and leave at -20oC ......閱讀全文
Ethanol－precipitation－method－for－purifying－PCR－products
1. For each PCR reaction (50 μL) prepare a 1.5mL microcentrifuge tube containing the following:? ?????? - 5 μL of 3M sodium acetate (NaOAc), pH 4.6?
Ethanol－precipitation－method－for－purifying－PCR－products
1. For each PCR reaction (50 μL) prepare a 1.5mL microcentrifuge tube containing the following:??????? - 5 μL of 3M sodium acetate (NaOAc), pH 4.6????
PEG－PRECIPITATION－OF－PCR－PRODUCTS
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CORE－SAMPLE－PCR:－A－method－to－rePCR－unique－bands－from－products－of－mixed－s
INTRODUCTION The products of a PCR reaction - especially when this is done on eukaryotic genomic DNA, and when using degenerate primers - often cont
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TRFLP的優缺點
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TRFLP技術的優缺點
T-RFLP（末端限制性片段長度多態性）該技術在應用的過程中，肯定需要在實驗條件上不斷進行改進，而這種改進的好壞自然而然需要實驗結果的驗證。V. Grüntzig在2002年做了該工作的一部分，結果認為，在限制性酶切時，很有必要去除其中影響DNA的酶切過程，并且實驗證明了具體的酶切時間。具有說服力的
Isolation－Of－PCR－Products
實驗概要 Rapid and efficient purification of PCR products from salts, primers, dNTPs, and other non-nucleic acid reagents. 實驗原理 The ChargeSwitch? Tech
DNA標記
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實驗概要The E.Z.N.A.? Tissue DNA Kit provides a rapid and easy method for the isolation of genomic DNA for consistent PCR and Southern analysis. Up to
酵母人工染色體
·?????????Easy YAC Preparation Method?(Andrew Davies,Shaw lab)·?????????Screening YAC libraries?(Donis Keller Lab)This is a method for screening YAC l
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Electrophoresis of PCR products with Life Technologies Sunrise gel apparatusGel:?In a 500 ml Pyrex? glass bottle, add:Agarose:3 gH2O270 mls10X TA30 ml
E.Z.N.A.?－Protocol－for－Tissue
實驗概要The E.Z.N.A.? ?Tissue DNA Kit provides a rapid and easy method for the isolation of ?genomic DNA for consistent PCR and Southern analysis. Up to 3
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實驗概要The E.Z.N.A.? Tissue DNA Kit provides a rapid and easy method for the isolation of genomic DNA for consistent PCR and Southern analysis. Up to
DNA－methyltransferase－Assay
Methylated CpG island Amplification?Protocol written by Minoru Toyota2. Materials2.1. MCARestriction enzymes SmaI, XmaIT4 DNA ligaseTaq DNA polymerase
RNA－Purification－from－1020－mg－Paraffinembedded－Tissue
實驗概要 The E.Z.N.A.? ?SQ Tissue RNA Kit is designed for isolating total RNA from animal ?tissue and cultured cells. The solution based system can be e
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實驗概要The E.Z.N.A.? ?Tissue DNA Kit provides a rapid and easy method for the isolation of ?genomic DNA for consistent PCR and Southern analysis. Up to 3
DNA的酶學操作
DNA的酶學操作DNA Modifying Enzymes?(Michael Blaber)Introduction to bacterial restriction/modification system. It provides very useful background knowledge
High－Throughput－Isolation－Of－PCR－Products－Using－ChargeSwitch?－PCR－CleanUp
實驗概要The ChargeSwitch? ?PCR Clean-Up Kit allows rapid and efficient purification of PCR ?products from salts, primers, dNTPs, and other non-nucleic aci
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General AdvicePCR allows the production of more than 10 million copies of a target DNA sequence from only a few molecules. The sensitivity of this tec
DNA提取中EB的去除實驗方法
Removal of Ethidium Bromide from DNA by Extraction with Organic SolventsJoseph SambrookPeter Maccallum Cancer Institute and The University of Melbourn
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General AdvicePCR allows the production of more than 10 million copies of a target DNA sequence from only a few molecules. The sensitivity of this tec
多克隆抗體
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SQ－Blood－DNA－Maxi－Protocol－for－410－ml－whole－blood
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SQ－Blood－DNA－Midi－Protocol－for－500l3ml－whole－blood
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