ProZyme公司推出GlycanQ系統用于綜合的N糖分析

　　分析測試百科網訊，位于加利福尼亞州海沃德的ProZyme公司，糖生物學領先的試劑和工具制造商，2016年11月14日宣布推出Glycan-Q糖分析系統, 一個集成的高通量平臺, 可進行用戶友好的糖基分析。Glycan-Q系統利用ProZyme在快速糖樣品制備和分析級糖基標準品方面的專長, 并結合小型毛細管凝膠電泳 (CGE) 儀器，后者裝載有為簡化糖數據分析而設計的定制化軟件。

　　"我們很高興推出ProZyme 的第一款儀器產品, " ProZyme 的總裁兼首席執行官Sergey Vlasenko博士說。"我們在過去幾年中加快了糖樣品制備的工作, 但數據分析仍然是一個瓶頸。通過我們的新系統, 生物制藥公司的細胞線開發和分析組可以更快地確定樣品的糖譜, 同時降低總體成本。我相信該系統可以幫助研究人員運行 glycomic（糖組學）分析的大型數據集, 包括臨床樣本。

　　蛋白質糖基化是一種翻譯后修飾, 可影響治療蛋白的有效性和安全性, 如對單克隆抗體, N-糖基化是一個關鍵的質量屬性。蛋白質糖基化也可以作為特定疾病狀態的指示。傳統的N-糖分析方法包括緩慢的、耗費大量人力的步驟來進行樣品制備、糖分離和數據分析。ProZyme 推出了Glycan-Q系統，實現了糖分析的高通量和簡單化，可用于生物治療藥物的開發, 并幫助發現糖生物標志物。

　　在Glycan-Q工作流的前端, ProZyme Gly-X化學酶和PNGase（聚糖酶）的作用下，可5分鐘內從糖蛋白中釋放N-糖，釋放出的N-糖立即用熒光染料標記, 然后用CGE分離。整個樣品準備過程可在45分鐘內完成, 并且是自動化的。熒光標記的N-糖然后在Glycan-Q CGE 儀器上分離, 每個樣本運行時間為2分鐘, 允許在一個8小時的工作日內準備和分析96個樣本。該儀器具有易于更換的Glycan-Q卡盒, 含有毛細管和凝膠基質。

　　Glycan-Q儀器控制和N-糖數據分析由定制構建的Glycan-Q管理器軟件完成。電泳標準品是與每個樣本共同注入的, 這與階梯標準品一起實現了對每個樣本峰值的自動數據對準和葡萄糖單位 (GU) 值的計算。然后將樣本的GU值與內置和可自定義的糖參考庫進行比較, 以進行峰值分配。通過 ProZyme 的糖標準品和外切糖苷（exoglycosidase）來增強分析效果。

　　Gly-Q系統用于N-糖樣品制備、分離和分析，由ProZyme 銷售。要了解更多關于Gly-Q系統的信息, 請訪問 prozyme.com:

　　Gly-Q系統糖分析系統

　　Gly-Q管理者軟件

　　Gly-X N-糖快速釋放，InstantQ 套件標記

關于ProZyme

　　ProZyme, Inc. 創立于1990年，其愿景是建立一家高品質、有附加值、客戶驅動的生化試劑公司。25年來，我們自豪地為世界各地的生物技術、生物制藥和學術機構提供最高水準的產品、服務和支持。ProZyme的旗艦產品線是我們的糖生物學產品陣容，以及鏈親和素、藻膽蛋白和偶聯物，我們還與我們的客戶合作開展定制分析服務。ProZyme恪守承諾，即開發、交付、支持糖生物學這一激動人心而迅速擴張領域中的各類產品。

　　英文原文：

　　ProZyme, Inc. Launch Gly-Q System For Integrated N-Glycan Analysis

　　Published: Nov 15, 2016

　　HAYWARD, Calif., Nov. 14, 2016 /PRNewswire-iReach/  --ProZyme, Inc., a leading manufacturer of reagents and tools for glycobiology, today announced the launch of the Gly-Q Glycan Analysis System, an integrated platform for high-throughput, user-friendly glycoanalysis. The Gly-Q System will leverage ProZyme's expertise in rapid glycan sample preparation and analytical glycan reference standards, combined with a small capillary gel electrophoresis (CGE) instrument powered by custom software for simplified glycan data analysis.

　　Photo  -http://photos.prnewswire.com/prnh/20161114/438816

　　"We are delighted to introduce ProZyme's first instrument offering," said Sergey Vlasenko, Ph.D., President and CEO of ProZyme. "We have led the way in speeding up glycan sample preparation in the past few years, but data analysis has still been a bottleneck. With our new system, cell line development and analytical groups at biopharma companies can determine the glycan profiles of their samples faster, while reducing overall costs. I believe this system could benefit researchers running glycomic analysis of large datasets including clinical samples."

　　Protein glycosylation is a post-translational modification that can influence the efficacy and safety of therapeutic proteins such as monoclonal antibodies, and N-glycosylation can be a critical quality attribute. Protein glycosylation can also serve as a signature of a specific disease state. Traditional methods for N-glycan analysis involve slow and labor-intensive steps for sample preparation, glycan separation and data analysis. ProZyme introduces the Gly-Q system to enable high-throughput and accessible glycan analysis for use in biotherapeutic development, and to aid in glycan biomarker discovery.

　　At the front end of the Gly-Q workflow, ProZyme Gly-X chemistry enzymatically releases N-glycans from glycoproteins with PNGase F in 5 minutes, and released N-glycans are instantly labeled with a fluorescent dye that enables separation by CGE. The entire sample preparation protocol can be completed in as little as 45 minutes, and is automatable. Fluorescently-labeled N-glycans are then separated on the Gly-Q CGE instrument with a run time of 2 minutes per sample, allowing 96 samples to be prepared and analyzed within a single 8-hour work day. The instrument features an easily-replaceable Gly-Q cartridge containing a capillary and gel matrix.

　　Gly-Q instrument control and N-glycan data analysis is facilitated by custom-built Gly-Q Manager software. Migration standards are co-injected with each sample, which along with a ladder standard enables automatic data alignment and the calculation of glucose unit (GU) values for each sample peak. GU values for samples are then compared against built-in and customizable N-glycan reference libraries for peak assignment. Analysis is enhanced with ProZyme's glycan reference standards and exoglycosidase enzymes.

　　The combined Gly-Q System for N-glycan sample preparation, separation and analysis is exclusively marketed by ProZyme worldwide. To learn more about the Gly-Q System, please visit prozyme.com:

　　Gly-Q Glycan Analysis System

　　Gly-Q Manager Software

　　Gly-X N-Glycan Rapid Release & Labeling with InstantQ Kit

　　About ProZyme

　　ProZyme, Inc. is a privately held company based in Hayward CA founded in 1990 with a vision of establishing a high-quality, value-added and customer-driven biochemical reagent company. We are proud, 26 years on, to provide the highest level of products, services and support to our customers at biotechnology, biopharmaceutical and academic institutions across the world. ProZyme's flagship product line is an extensive glycobiology portfolio, alongside streptavidin, phycobiliprotein and conjugate offerings. We perform custom analytical services, and welcome the opportunity to collaborate with our customers. ProZyme maintains a commitment to develop, release and support products in the exciting and rapidly-expanding area of glycobiology.

　　Media Contact:Aled Jones, ProZyme, Inc., (510) 638-6900, info@prozyme.com

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　　SOURCE ProZyme, Inc.