DNA的誘變和甲基化

·         In Vitro Mutagenesis Using Altered Sites (Bowtell Lab) 

• In vitro Mutagenesis with dut ung single stranded DNA (Hahn Lab)

·         Site-directed Mutagenesis using PCR (Lazo Lab)
PCR site-directed methods allow site-specific mutations to be incorporated in virtually any double-stranded plasmid; eliminating the need for M13-based vectors or single-stranded rescue.

·         Site-directed mutagenesis (Dr. Chastain)

·          Site Directed Mutagenesis Using the Ung-Dut System (Bowtell Lab)

·         Oligonucleotide-directed mutagenesis (Kunkel method) (Jeff NEwman)

·         Site-directed Mutagenesis (Promega)
For generation and selection of oligonucleotide-directed mutants

·         Site Directed Mutagenesis Using the Ung-Dut System (PMCI Research)

·         Nested Deletions (Bowtell Lab)
Nested Deletions using exonuclease-III and mung bean nuclease

·         Deletion Mutagenesis (Promega)
For construction of plasmid or M13 subclones containing progressive unidirectional deletions of  inserted DNA, Including methods for DNA preparation, vector digestion, exonuclease deletion, ligation,  transformation...

·         ExoIII/S1 Deletion (Fermentas)
For construction of plasmid or M13 subclones with nested unidirectional deletions. The method is applicable for DNA sequencing, mapping of boundaries of regions involved in genetic control, DNA/protein interaction sites, or protein domains.

·         Exo/S1 Deletion Series (Vesicle Trafficking)

Four Primer Mutagenesis (PDF) (Templenton Lab)
A PCR-based mutagenesis, based on Sarkar and Sommer, but with no ligation required. 

·         Nested Deletions Using Exonuclease-III and Mung Bean Nuclease (PMCI Research)

·         In vitro Mutagenesis Using Altered Sites (PMCI Research)

·         Unidirectional deletions (erase-a-base) (Crawford Lab)

Design of Mutagenic Oligonucleotides (Promega)

Methylation analysis

·         Bisulfite Treatment of DNA (Issa Lab)

·         Methylation-Sensitive PCR (MSP) (Issa Lab)

·         Bisulfite-PCR (for restriction and/or sequencing) (Issa Lab)

·         Southern Blot Analysis (Issa Lab)

·         Methylated CpG Island Amplification (MCA) (Issa Lab)

·         DNA-Methyltransferase Assay (Issa Lab)

·         Bisulfite Modification (TTO)
Modify DNA with bisulfite for methylation analysis